Figure 6.

Effect of ZNT7 knockdown on the CD40 signaling pathways in Raji B lymphocytes. ZNT 7 KD and control Raji B cells were seeded and cultured for 48 h. CD154 stimulation (100 ng·mL⁻¹) was carried out at 0, 5, 10, or 20 min at 37 °C in duplicate. After stimulation, the cells were washed and lysed for western blot analysis. (A) Phosphorylation of p38 MAPK. (B) Phosphorylation of AKTs. Total p38 MAPK and AKTs were also examined. (C) Phosphorylation of IKKs. ACTB was used for the loading control. The activations of STAT3 and ERK1/2 signaling pathways were not detectable in both ZNT7 KD and control Raji B cells after CD154 stimulation (data not shown). Experiments were each performed twice and representative results are shown. (D) The CD154‐CD40 signaling pathways in B lymphocytes. CD154‐stimulated CD40 signaling activates multiple downstream signaling pathways in B lymphocytes that influence B lymphocyte proliferation, isotype switching, differentiation, and survival. The diagram represents the pathways that were examined in this study. Single arrow, direct stimulation; multiple arrows, multistep simulation; p with a circle, activated by phosphorylation.