Figure 7.

Effect of ZNT7 OE on the activations of p38 MAPK and AKTs and cell surface expression of CD40 in Raji B cells. Raji B cells overexpressing ZNT7 were stimulated with CD154 (100 ng·mL⁻¹) at 37 °C for 5–10 min before harvesting. Western blot analysis was performed as described in Materials and methods. The expression of β‐Tubulin or BIP was used as the loading control. (A) Activation of p38 MAPK in ZNT7‐overexpressing Raji B cells. (B) Ratio of phosphor‐p38 MAPK to the total p38 MAPK. (C) Activation of AKTs in ZNT7‐overexpressing Raji B cells. (D) Ratio of phosphor‐AKTs to the total AKTs. (E) Cell surface expression of CD40 (indicated by the levels of the CD40‐bound IgG chains). (F) Densitometry analysis of the CD40‐bound IgG heavy chain. The amount of expressed BIP was used for the normalization. Experiments were each performed twice with duplication and representative western blots are shown. *P < 0.05; **P < 0.01.