Figure 2.

Generation of transgenic ramie plants. (A) 40‐day‐old plants grown on MS medium supplemented with 0.01 mg·L⁻¹ NAA were used for leaf midrib explant preparation. The triangular flask is 6 cm in diameter and 10 cm in height. (B) Leaf midrib explants were infected with EHA105 harboring pSG529 and cultured on solid cocultivation medium (MS medium + 0.2 mg·L⁻¹ TDZ + 0.04 mg·L⁻¹ 2,4‐D + 50 mg·L⁻¹ AS). The diameter of Petri dish is 9 cm. (C) After 2 days’ coculture, leaf midrib explants were incubated on selection medium (MS + 0.2 mg·L⁻¹ TDZ + 0.04 mg·L⁻¹ 2,4‐D + 40 mg·L⁻¹ kanamycin+750 mg·L⁻¹ cefotaxime). (D) Infected leaf midrib explants on selection medium produced Km‐resistant shoots after 3 weeks of selection culture. (E) Transgenic plants were cultured on elongation medium (MS medium + 250 mg·L⁻¹ cefotaxime + 0.01 mg·L⁻¹ NAA). (F) Transgenic ramie plants rooted. (G) Transgenic shoots propagated on MS medium supplemented with 0.01 mg·L⁻¹ NAA. (H) Transgenic plants grown in Hoagland's solution. The cylindrical bottle is 4 cm in diameter and 10 cm in height. (I) Transgenic ramie plants were transplanted to small plastic pots in the greenhouse. The length, width, and height of the pots are 11, 11, and 14 cm, respectively.