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. Author manuscript; available in PMC: 2017 Apr 28.
Published in final edited form as: Cell Rep. 2017 Mar 7;18(10):2452–2463. doi: 10.1016/j.celrep.2017.02.034

Fig.2.

Fig.2

Evaluation of interaction between the Rab4-assocated vesicles and Kinesin motors in tissue cultured cells using the FRB-FKBP assay.

A) Constructs used in the FRB-FKBP inducible interaction assay to assess the interaction between Rab4 associated vesicles and the stalk-tail domains of different kinesins.

B) Schematic of the FRB-FKBP interaction assay with expected readouts in the case of a positive or negative interaction.

C) The localization of Emerald-Rab4, FRB bait proteins, and tdTomato-BicD2-FKBP in control fibroblasts and in the fibroblasts following the addition of the linker. Linescans from the centroid of the nucleus (dashed lines in Merge) show the degree to which the intensity profiles of Bicaudal and Rab4 overlap. See also Figure S2.