Figure 2. TNF enhances SAA1/2 expression in ovarian cancer. (A) Expression levels of TNF mRNA determined by real-time PCR in five different ovarian normal (N1-N5) and tumor tissues (T1-T5). (B) Correlation between mRNA levels of TNF and SAA isoforms. (C) Time-dependent effects of TNF on SAA1/2 mRNA by real-time PCR in ovarian cancer cell lines (OVCAR-3 and SKOV-3). Cells were incubated with TNF (10 ng/ml) for 0, 1, 3, 6, 12 and 24 hours. ^*Indicates significant increase (p≤0.05) compared to its own control as calculated by the paired Student's t test.