FIGURE 6.

Overexpression of STAT3C diminished the cytotoxic effect of AR in CRC cells. HCT-116 (A) and SW480 (B) cells were transiently transfected with either the STAT3C-expressing construct or the empty vector for 48 h. Protein expression levels were examined by immunoblotting. After plasmid transfection, HCT-116 (C) and SW480 (D) cells were treated with various concentrations of AR for 48 h, and the cell viability was determined by the MTT assay. Data were the percentages of cell viability of individual AR treatment groups against that of corresponding control groups (in which cells were transfected with the empty vector and STAT3C-harbing plasmid, respectively) but not treated with AR, and were shown as mean ± SD from three independent experiments. ^∗p < 0.05, ^∗∗p < 0.01.