Fig. 6.

ELS alters the developmental expression of markers of neuronal proliferation and differentiation similarly in male and female hippocampus. Gene expression for Ki-67 in (a) male and (b) female whole hippocampus as well as doublecortin (DCX) in (c) male and (d) female whole hippocampus. Gene expression was assessed by realtime qPCR and calculated relative to 18S. We observed a significant decrease in expression over development, and observed lower levels of expression in ELS reared hippocampus at early developmental ages for both sexes and both markers, but no significant effects of sex or treatment beyond P28. *significant main effects ^#significant post-hoc (Tukey LSD). Alpha was set at (p < 0.05). Data represent the group mean (+/−SEM).