Figure 2.

Gel filtration chromatography of Vip3Aa treated with trypsin. Vip3Aa was incubated with trypsin (24:100 trypsin:Vip3A, w:w) for 30 min (“Input” in figure inset). The sample was loaded into a Superdex-75 10/300 GL column and elution fractions (1 mL each) were analysed by SDS-PAGE (“Output” in figure inset). Molecular weight markers (M) are indicated in kDa.