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. 2017 Apr 7;9(4):131. doi: 10.3390/toxins9040131

Figure 4.

Figure 4

Trypsin processing of Vip3Aa in the presence of sodium dodecyl sulfate (SDS) and β-mercaptoethanol. Vip3Aa was treated with trypsin (24:100 trypsin:Vip3A, w:w) with or without SDS or β-mercaptoethanol, incubated for 30 min at 30 °C and subjected to SDS-PAGE. Lane 1: molecular weight markers; lane 2: control without SDS or β-mercaptoethanol; lane 3: with SDS; lane 4: with β-mercaptoethanol; lane 5: with SDS and β-mercaptoethanol; lane 6: Vip3Aa without trypsin (protoxin). All reactions were stopped with 1 mM AEBSF followed by 10 min incubation at room temperature (RT). Molecular weight markers (kDa) are indicated in the left. The band of around 23 kDa corresponds to trypsin.