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Gel filtration chromatography of Vip3Aa treated with A. ipsilon midgut juice (MJ). Vip3Aa was incubated with 40:100 MJ:protein (w:w) for 1 h (“Input” in figure inset). The sample was loaded into the column and the elution fractions (1 mL each) were analysed by SDS-PAGE. The protein profile of the output (fraction A1) is shown in the figure inset. Molecular weight markers (MW) are indicated on the right in kDa.
