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Molecular weight of the subunits of Stx1, Stx2 and ricin (a) and SDS-PAGE analysis (b); Stx1 and Stx2 (7 µg) were treated with trypsin with or without TCEP, and ricin (7 µg) was treated with TCEP at 25 °C for 2 h and separated on a 12% SDS-PAGE. The gel was stained with Coomassie blue. The protein marker is Invitrogen Novex® sharp pre-stained protein marker. * Molecular weight is affected by glycosylation in both A and B subunits of ricin.
