Table 5.
Primers used in the PCR analysis of cry1, cry2, cry9, vip3, chi36, and chit genes.
| Target Gene Family | Product Size (pb) | Primers Set | Sequence (5′ → 3′) | Tm a (°C) | Reference |
|---|---|---|---|---|---|
| cry1 | 274–277 | Un1(f) | CATGATTCATGCGGCAGATAAAC | 67.2 | [55] |
| Un1(r) | TTGTGACACTTCTGCTTCCCATT | 66.7 | |||
| cry2 | 689–701 | Un2(f) | GTTATTCTTAATGCAGATGAATGGG | 63.3 | [55] |
| Un2(r) | CGGATAAAATAATCTGGGAAATAGT | 61.1 | |||
| cry9 | 354 | Un9(f) | CGGTGTTACTATTAGCGAGGGCGG | 71.5 | [55] |
| Un9(r) | GTTTGAGCCGCTTCACAGCAATCC | 73.3 | |||
| endochitinase | 1997 | Chit(f) | ATTCACACTGCTATTACTATC | 50 | [56] |
| Chit(r) | TGACGGCATTTAAAAGTTCGGC | 68.7 | |||
| exochitinase 36 | 1083 | Chi36(f) | GATGTTAAACAGGTTCAA | 50.2 | [12] |
| Chi36(r) | TTATTTTTGCAAGGAAAG | 52.9 | |||
| vip3 | 1395 | vip3-sc(f) | TGCCACTGGTATCAARGA | 54.2 | [57] |
| vip3-scII(r) | CCATTAATYGGAKTCAAAAATGTTTCACTGAT | 71.1 | The current work |
a Melting temperature.