Figure 2.

Effects of phloroglucinol on glucose production in mouse primary hepatocytes. (a) Mouse primary hepatocytes were treated with the indicated concentrations of phloroglucinol for 24 h and cell viability was determined by CCK-8 assay. Data are presented as means ± standard deviation (SD), three independent experiments were performed; (b) Mouse primary hepatocytes were treated with the indicated concentrations of phloroglucinol in the absence or presence of 10 nM glucagon and gluconeogenic substrates (2 mM pyruvate and 20 mM sodium lactate). Glucose production was measured by a glucose assay kit. Data are presented as means ± standard deviation (SD), five independent experiments were performed. (*** p < 0.001 vs. Vehicle, + p < 0.05 or ++ p < 0.01 vs. 10 nM glucagon; one-way ANOVA).