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. 2017 Apr 5;15(4):106. doi: 10.3390/md15040106

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effects of compound C (AMPKα inhibitor) on phloroglucinol-induced suppression of PEPCK and G6Pase gene expression in HepG2 cells. Cells were treated with 10 μM of compound C and 50 μM of phloroglucinol for 6 h in high glucose DMEM. (a) Protein bands were detected by Coomassie blue staining (left) and phospho-AMPKα (Thr-172), AMPKα, PEPCK and G6Pase protein levels were analyzed by Western blotting (right) and (b) PEPCK and G6Pase mRNA expression levels were analyzed by RT-qPCR. Data are presented as means ± standard deviation (SD), three independent experiments were performed. (*** p < 0.001 vs. vehicle or ^†† p < 0.005 vs. phloroglucinol; Student’s t-test).