Figure 4.

The effect of treatment with STC-1 on the expression of adipogenic-specific and AMP-activated protein kinase (AMPK) phosphorylation in 3T3-L1 adipocytes. The 3T3-L1 preadipocytes were incubated in differentiation medium without or with the indicated concentrations of STC-1 for 8 days (from day 0 to day 8). The expression of sterol regulatory element-binding protein 1c (SREBP-1c), peroxisome proliferator-activated receptor-γ (PPARγ), CCAAT/enhancer-binding protein α (C/EBPα) and AMPK was assessed by Western blotting as described in the Materials and Methods. Data are expressed as the means ± SE (n = 3). Significant differences from the control group were identified at * p < 0.05.