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Evaluation of reference (positive) extracts for the identification of target inhibitors by using affinity chromatography. Affinity chromatography profiles of the TCA-clarified extract of: P. homomalla (A); and the heat-clarified extract of X. muta (B) with HiTrap™ Plm II-Sepharose HP resin. Affinity chromatography profiles of the heat-clarified extracts of: P. homomalla (C); and S. helianthus (D) with Papain-Sepharose resin. Arrows indicate the addition of elution buffer.
