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. 2017 Apr 21;15(4):123. doi: 10.3390/md15040123

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Confirmatory experiments using binding assays for molecules able to interact with Plm II in the selected extracts. Affinity chromatography profiles of extracts: P31 (A); and P32 (B) with a HiTrap™ Plm II-Sepharose HP resin. Arrows indicate the addition of elution buffer. (C) Sensorgrams from the SPR biosensor-based assay for the interaction of extracts P11, P31 and P32 with Plm II. (D) Titration curve of purified PhPI with (pepstatin A-titrated) Plm II used to determine the concentration of active inhibitor. (E) Concave dose–response curve for the estimation of $K_{i}^{a p p}$ of PhPI with Plm II.