Table 1.
Names and Phylum distribution of the species included in this study. The identifiers (F: Falcipain 2; P: Plasmepsin II) used to distinguish each extract are indicated. The clarification treatment used in all cases was heating at 60 °C for 30 min unless otherwise indicated. Those extracts causing reduction levels equal or higher to 75% (hits) during primary screening are indicated by (+), and those further validated by interaction-based assays are indicated by (++). w.b.: whole body; s.: spikes; g.: gonads; b: body; b.c.: branchial crown; p.c.: polyps and coenenchyme; —: not tested.
| Species | Phylum | Identifier | |
|---|---|---|---|
| Falcipain 2 | Plasmepsin II | ||
| Hermodice carunculata | Annelida | F30 (+) | — |
| Sabellastarte magnifica (b.c.) | Annelida | F22 | — |
| Sabellastarte magnifica (b.) | Annelida | F23 | — |
| Bugula plumosa | Bryozoa | F39 | P25 |
| Copidozoum sp. | Bryozoa | — | P24 (+) |
| Zoobotrium sp. | Bryozoa | — | P30 |
| Zoobotrium sp. (TCA clarified) | Bryozoa | — | P33 |
| Ascidia sydneiensis | Chordata | F31 | — |
| Ascidia sydneiensis (TCA clarified) | Chordata | F44 | P28 |
| Diplosoma listerianum | Chordata | F33 | — |
| Ecteinascidia turbinata | Chordata | F28 | — |
| Microcosmus sp. | Chordata | — | P15 |
| Phallusia nigra | Chordata | F21 | — |
| Phallusia nigra (TCA clarified) | Chordata | F45 | — |
| Acetabularia sp. | Chlorophyta | — | P17 |
| Bryopsis pennata | Chlorophyta | — | P21 |
| Halimeda opuntia | Chlorophyta | F34 | — |
| Penicillus capitatus | Chlorophyta | — | P9 |
| Bartholomea annulata | Cnidaria | — | P11 (++) |
| Bunodosoma granulifera | Cnidaria | F11 (+) | P8 |
| Bunodosoma granulifera (TCA clarified) | Cnidaria | F32 (++) | — |
| Cassiopeia xamachana | Cnidaria | — | P29 |
| Condylactis gigantea | Cnidaria | F3 (+) | — |
| Condylactis gigantea (TCA clarified) | Cnidaria | F29 | — |
| Eunicea calyculata | Cnidaria | — | P13 |
| Lenubrea danae (p.c.) | Cnidaria | F16 | — |
| Lenubrea danae (w.b.) | Cnidaria | F17 | — |
| Lenubrea danae (w.b.) (TCA clarified) | Cnidaria | F36 (+) | — |
| Linuche unguiculata | Cnidaria | F15 (+) | — |
| Palythoa caribbaeorum | Cnidaria | F2 (+) | P5 |
| Palythoa caribbaeorum (TCA clarified) | Cnidaria | F12 (+) | — |
| Physalia physalis | Cnidaria | F47 | — |
| Plexaura homomalla | Cnidaria | F8 (++) | P31 (++) |
| Stichodactyla helianthus | Cnidaria | F6 (++) | P3 |
| Stichodactyla helianthus (TCA clarified) | Cnidaria | F38 (+) | P35 |
| Zhoanthus pulchelus | Cnidaria | F7 (+) | P22 |
| Zhoanthus sp. | Cnidaria | — | P16 |
| Echinaster echinophorus | Echinodermata | F35 | P4 |
| Echinometra lucunter | Echinodermata | — | P23 |
| Eucidaris tribuloides | Echinodermata | F24 (+) | — |
| Holothuria mexicana | Echinodermata | F42 (++) | P6 |
| Holothuria sp. | Echinodermata | F37 | — |
| Holothuria sp. | Echinodermata | — | P18 |
| Holothuria sp. | Echinodermata | — | P19 |
| Holothuria sp. | Echinodermata | — | P20 |
| Isostochopus badionotus | Echinodermata | F26 (+) | — |
| Luidia senegala | Echinodermata | — | P26 |
| Lytechinus variegatus (w.b.) | Echinodermata | F40 | — |
| Oreaster reticulates | Echinodermata | — | P27 |
| Tripneustes ventricosus (s.) | Echinodermata | F4 | — |
| Tripneustes ventricosus (g.) | Echinodermata | F5 (+) | — |
| Tripneustes ventricosus (w.b.) | Echinodermata | F10 | P2 |
| Cenchritis muricatus | Mollusca | F43 (+) | P7 (+) |
| Nerita peloronta | Mollusca | F20 (++) | — |
| Nerita versicolor | Mollusca | F41 (+) | — |
| Purpura patula | Mollusca | — | P12 |
| Ectyoplasia ferox | Porifera | — | P32 (++) |
| Lissodendorix isodyctialis | Porifera | F25 (+) | P1 |
| Xetospongia muta | Porifera | F46 | P10 * |
| Xetospongia muta (TCA clarified) | Porifera | — | P34 |
| Galaxaura sp. | Rhodophyta | F27 (+) | — |
| Laurencia sp. | Rhodophyta | — | P14 |
* False-negative extract validated by affinity chromatography.