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. 2017 Apr 21;5(8):e13259. doi: 10.14814/phy2.13259

Figure 2.

Figure 2

Exposure to hypertonic saline causes a dose‐dependent, time‐dependent, reversible decrease in immunoreactivity to PIP2 in the plasma membrane of isolated MNCs. (A) The bar graph shows the mean normalized immunoreactivity to PIP2 in MNCs maintained in isotonic saline or hypertonic saline of 305, 315, 325, and 345 mosmol kg−1 for 5 min. (B) The bar graph shows the mean normalized immunoreactivity to PIP2 in MNCs maintained in isotonic saline or exposed to hypertonic saline (325 mosmol kg−1) for the indicated times. (C) The bar graph shows the mean normalized immunoreactivity to PIP2 in MNCs maintained in isotonic saline, after 5 min in hypertonic saline (325 mosmol kg−1), and after 5 min in hypertonic saline and 5 min in isotonic saline. The values for the “hypertonic & recovery” MNCs were compared to those of the “hypertonic” MNCs. Data are expressed as mean normalized fluorescence intensity ± SEM (P < 0.05 is indicated by *; P < 0.01 by **).