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. 2017 Apr 21;5(8):e13259. doi: 10.14814/phy2.13259

Figure 3.

Figure 3

The osmotically‐evoked decrease in membrane PIP2 depends on the activation of TRPV1 channels and on extracellular Ca2+. (A) The bar graph shows the mean normalized immunoreactivity to PIP2 in MNCs maintained in isotonic saline, exposed to hypertonic (325 mosmol kg−1) saline for 5 min, and exposed to hypertonic saline in the presence of the TRPV1 channel antagonist SB366791 (5 μmol/L) for 5 min. (B) The bar graph shows the mean normalized immunoreactivity to PIP2 in MNCs maintained in isotonic saline, exposed to hypertonic (325 mosmol kg−1) saline for 5 min, and exposed to hypertonic saline that contains no added Ca2+ for 5 min. Data are expressed as mean normalized fluorescence intensity ± SEM (P < 0.05 is indicated by *; P < 0.01 by **).