Table 1.
Antibodies used for western blot analysis
| Antibodies | Primary dilution | Secondary dilution | Company/Product no. |
|---|---|---|---|
| Cu‐Zn superoxide dismutase (SOD1) | 1:3000 | 1:3000 | Abcam: ab16831 |
| Mn superoxide dismutase (SOD2) | 1:5000 | 1:3000 | Abcam: ab13533 |
| Catalase (CAT) | 1:1000 | 1:3000 | Sigma Aldrich: C0979 |
| Cytochrome C (CYT C) | 1:1000 | 1:3000 | Cell Signaling Technology: 4280 |
| Voltage‐dependent anion channel (VDAC) | 1:500 | 1:1000 | Cell Signaling Technology: 4661 |
| Cytochrome c oxidase IV (COX IV) | 1:500 | 1:1000 | Cell Signaling Technology: 4850 |
| Succinate dehydrogenase (SDHA) | 1:1000 | 1:2000 | Cell Signaling Technology: 11998 |
| Prohibitins 1 (PHB1) | 1:1000 | 1:2000 | Cell Signaling Technology: 2426 |
| Acetyl‐Histone H3 (Lys9) | 1:1000 | 1:2000 | Cell Signaling Technology: 9649 |
| Peroxisome proliferator‐activated receptor gamma coactivator 1 alpha (PGC1α) | 1:1000 | 1:2000 | Abcam: ab72230 |
| Estrogen‐related receptor alpha (ERR α) | 1:1000 | 1:2000 | Cell Signaling Technology: 13826 |
| Sirtuin 1 (SIRT1) | 1:500 | 1:2000 | Millipore: 07‐131 |
| Transcription factor A, mitochondrial (TFAM) | 1:375 | 1:1000 | Cell Signaling Technology: 8076 |
| Total OXPHOS rodent WB antibody cocktail | 1:1000 | 1:10000 | Abcam: ab110413 |
| Binding immunoglobulin protein (BIP) | 1:700 | 1:2000 | Cell Signaling Technology: 3177 |
| Protein kinase RNA‐like endoplasmic reticulum kinase (PERK) | 1:700 | 1:2000 | Cell Signaling Technology: 5683 |
| Inositol‐requiring enzyme 1 (IRE1α) | 1:700 | 1:2000 | Cell Signaling Technology: 3294 |