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. Author manuscript; available in PMC: 2017 Oct 27.
Published in final edited form as: Oncogene. 2016 Oct 31;36(17):2435–2445. doi: 10.1038/onc.2016.399

Figure 2. HBV infection induces DNA demethylation of SALL4 in HepG2hNTCP cell line.

Figure 2

(a) Quantification of secreted HBeAg by ELISA (Left panel), and quantification of pregenomic RNA by QPCR (Right panel) after HBV infection for 6 days. (b) Quantification of SALL4 gene expression (SALL4A and SALL4B slicing variants) by QPCR after HBV infection for 4 and 6 days (D4, D6). (c) Bisulfite sequencing PCR results of SALL4 clones, using DNA from HepG2hNTCP cells without (−) HBV infection (D0) or with (+) HBV infection at 4 and 6 days (D4 and D6). Open and closed circles denote unmethylated and methylated states, respectively. Error bars denote S.D. *P< 0.05; **P< 0.01.