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. Author manuscript; available in PMC: 2017 Oct 27.
Published in final edited form as: Oncogene. 2016 Nov 7;36(17):2446–2456. doi: 10.1038/onc.2016.400

Figure 5. Inhibition of mTORC1 signaling by rapamycin in CAMK2γ−/− mice.

Figure 5

CAMK2γ−/− mice and WT mice received either 5mg/kg rapamycin or 2% vehicle i.p. injection 2 h before the DEN challenge. The mice then received a second and a third rapamycin or vehicle injection 24 h and 46 h after the DEN challenge. At 48 h after the DEN injection, the mice were sacrificed and the liver were harvested for PCNA immunostaining and Western blotting analysis. (A) Immunoblotting analyses of mTORC1 signaling in the liver 48 h after DEN treatment. Small arrow indicates the mTOR band. (B) Representative images of PCNA staining in the liver 48 h after DEN treatment. (C) Quantification of proliferating hepatocytes by PCNA staining. (D) Quantification of hepatic inflammatory cell infiltration, centrilobular necrosis, and hepatocyte apoptosis. **, p <0.01; n=4–7.