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. 2017 Apr 1;3(4):302–308. doi: 10.1021/acscentsci.6b00391

Figure 6.

Figure 6

Substrate-selective cyclopropanation catalyzed by a variant of CYP119 in comparison to the same reaction catalyzed by the free cofactor. Reaction conditions: 10 mM 25, 10 mM 26, 60 mM EDA (added by syringe pump over 1 h), 0.17% catalyst, 100 mM NaPi/100 mM NaCl, pH 6.0, 3 vol % DMF. The amounts of 11 and 15 are the sum of all stereoisomers of the product. In the case of the enzyme catalyzed reaction, 11 and 15 were produced in comparable stereoselectivities to those of reactions of the individual substrates.