Skip to main content
. 2017 Jan 10;118(6):1432–1441. doi: 10.1002/jcb.25802

Figure 3.

Figure 3

Runx1 protects lymphoma cells against dexamethasone‐mediated apoptosis. (A) p53 null lymphoma cells transduced with the pBabeRunx1 retroviral vector or the pBabePuro vector control (p/m97 shown) were plated in triplicate in the presence and absence of 1.0 μM dexamethasone and monitored for live/dead counts by trypan blue exclusion over 4 days. (B) The same cells were plated at 4 × 105 per well in triplicate wells of a 12‐well plate and monitored for growth over 48 h. Ectopic Runx1 significantly reduced cell proliferation at 24 h (P < 0.01), 40 h (P < 0.01), and 48 h (P < 0.01). (C) qt‐RT‐PCR analysis of Runx1, Sgpp1, Ugcg, and Nr3c1 in pBabePuro vector control and pBabeRunx1‐expressing T lymphoma cells (p/m97 shown) grown in the presence and absence of 1.0 μM dexamethasone for 6 h. The data are means ± SD where n = 9 representing three technical replicates of each biological replicate (3) from one experiment typical of two.