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. 2017 Jan 10;118(6):1432–1441. doi: 10.1002/jcb.25802

Figure 5.

Figure 5

shRNA knockdown of Sgpp1 reduces dexamethasone‐mediated apoptosis. (A) qt‐RT‐PCR analysis of Sgpp1 expression in p/m97 cells stably infected with viral supernatants expressing an Sgpp1 or a non‐coding (NC) control shRNA sequence. Cells were grown for 6 h in the presence of 1.0 μM dexamethasone prior to RNA extraction and qt‐RT‐PCR analysis. The data were calculated as described in Fig. 4C. (B) The same cells were plated in triplicate and grown for 36 h in the presence (Dex) and absence (MtOH) of 1.0 μM dexamethasone and monitored for live/dead counts by trypan blue exclusion. Knockdown of Sgpp1 had no effect on cell viability under control conditions but gave a significant reduction in cell death in the presence of 1.0 μM dexamethasone. (C) Interplay between Runx1 and dexamethasone on the expression of sphingolipid metabolism enzymes involved in the synthesis and breakdown of sphingosine and ceramide and their potential contributions to cell death and survival [Bianchini et al., 2006; Kilbey et al., 2010].