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. 2017 Apr 27;17:39. doi: 10.1186/s12896-017-0359-0

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Results from media screen for tissue culture of P. hallii. a Effect of media on germination. Data represent three replicates of 33 seeds per plate. b Effect of media type on callus induction. c Types of callus from each medium. d Regeneration effect scored as shoots per callus piece for callus induced on each medium. Data represent three replicates of one gram each of callus (7–11 pieces). e Callus proliferation measured in grams for each medium. Data represent three replicates of two grams of callus. a, b, d, e) Populations were analyzed separately under a one-way ANOVA controlling for medium. ANOVA tests showed differences among treatments for both HAL2 and FIL2 (p < 0.01). Mean separation was analyzed with Tukey’s HSD, error bars represent the standard error of the mean