Fig. 2.

Hairpin library design. a A total of 281 Kmers were designed, with 202 Kmers targeting a unique gene of N. benthamiana annotated genome with 100% identity, and 79 kmers targeting two or more genes. b To produce multiple gene silencing constructs, groups of six kmers were synthesized in tandem (4 “singles” and 2 “multiple”), cloned in a Gateway-enabled pUC57 vector and eventually recombined by LR reaction into the hairpin-producing destination vector pTKO2