Fig. 8.

GCNF induced significant dose and time dependent increase in ROS production depicted by the increase in DCFDA fluorescence measured through plate reader (a) and respective fluorescence images (b). Scale Bar – 300 μm. Per view 100 cells and 4 views per group were analyzed. (c ) Effect of GCNF on lipid peroxidation (LPO) was monitored compared to control cell. (d, e) NAC (ROS scavenger) have significant impact on viability of GCNF exposed A549 cells as well as ROS production in GCNF exposed A549 cells. Values are expressed as mean ± SE of three independent experiment. *p<0.05 was considered as statistical significant. (f) Immunoblotting analysis of apoptotic as well as autophagic protein in presence and absence of NAC after GCNF exposure in A549 cell. GAPDH was used as an internal control. Densitometry analysis is represented by the digits given above the respective blots and also provided in Additional file 7: Figure S7. Values are expressed as mean ± SE of three independent experiment. *p<0.05 was considered as statistical significant