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. 2017 Apr 27;18:12. doi: 10.1186/s12867-017-0089-9

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Representative plots of mouse ß-actin specific real-time PCRs. PCRs using mß-actin_SD primer pair amplified mß-actin_T (Ct = 15.76) and mγ-cyto-actin_T (Ct = 26.94) DNA (b, c), but did not amplify mα-SMA_T and mγ-smooth-actin_T DNA fragments (a, d). Our mouse ß-actin PCR resulted in products with melting peaks at 84.8 and 83.3 °C (e) and a discrete band at 106 bp in agarose gel after electrophoresis (f)