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. 2017 Apr 27;18:12. doi: 10.1186/s12867-017-0089-9

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 7 — Representative plots of real-time PCRs using literary primer pairs specific for mouse α-SMA. Real-time PCRs using mα-SMA_L1, mα-SMA_L2 or mα-SMA_L3 primer pairs and mα-SMA_T DNA templates resulted in products (Ct_L1 = 28.09, Ct_L2 = 17.19, Ct_L3 = 16.79) with single melting peaks at 82, 81.9, or 83.3 °C and in discrete bands in the agarose gel at 60, 101 or 154 bp, respectively (a–c). Real-time PCRs using mα-SMA_L1, mα-SMA_L2 or mα-SMA_L3 literary primer pairs amplified the non-specific mß-actin_T DNA templates also (Ct_L1 = 31.09, Ct_L2 = 34.62, Ct_L3 = 32.96), resulted in melting peaks at 81, 81.9, or 83.3 °C and in discrete bands at 60, 101 or 154 bp, respectively (a–c)