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. 2017 Apr 27;18:12. doi: 10.1186/s12867-017-0089-9

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 8 — Representative plots of real-time PCRs using literary primer pairs specific for mouse ß-actin. Real-time PCRs using mß-actin_L1, mß-actin_L2 or mß-actin_L3 primer pairs and mß-actin_T DNA templates resulted in products (Ct_L1 = 19.73, Ct_L2 = 27.72, Ct_L3 = 18.65) with single melting peaks at 85.6, 86.3, or 87.4 °C and in discrete bands in the agarose gel at 60, 101 or 154 bp, respectively (a–c). Real-time PCRs using mß-actin_L1, mß-actin_L2 or mß-actin_L3 literary primer pairs amplified the non-specific mα-SMA_T DNA templates also (Ct_L1 = 31.29, Ct_L2 = 21.08, Ct_L3 = 28.43), resulted in melting peaks at 85.6, 84.2 or 87.4 °C and indiscrete bands at 60, 101 or 154 bp, respectively (a–c)