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. 2017 Apr 27;18:12. doi: 10.1186/s12867-017-0089-9

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 9 — Comparison the results of real-time RT-PCRs investigating the mRNA expression of α-SMA or ß-actin using our or literary primer pairs. Depending on the applied primer pairs, the increase in mRNA expression of α-SMA varied from 3.1- to 6.2-fold in the kidneys of mice underwent UUO compared to the corresponding controls (a). While using our primer pair there was no difference in the mRNA expression of ß-actin, using literary primer pairs increased ß-actin mRNA expression was detected in the kidney of mice underwent UUO compared to the corresponding controls (b). Results were determined by comparison with RN18S and presented as mean + SD. *p < 0.05 UUO vs. control