Fig. 1.

A combined HPLC-CAD and LC-MS/MS workflow for total lipid profiling of larval zebrafish. Once an HPLC method was established that resolved lipids of all classes present in larval zebrafish, the instrument was temporarily reconfigured to route sample flow to a quadrupole-Orbitrap mass spectrometer instead of the charged aerosol detector. Data obtained from a series of LC-MS/MS experiments with exact mass detection was then used to identify the components of peaks observed by HPLC-CAD/fluorescence. Through this combined workflow, peak composition data from a single LC-MS/MS experiment greatly enhances the depth of information that may be obtained from all future HPLC experiments performed using the same instrument method.