Fig. 8.

Ezetimibe reduces CE synthesis from dietary FAs in the intestine. Larval zebrafish (6 dpf) were treated with ezetimibe (or 0.1% ethanol vehicle) and then fed BODIPY FL-C12 in the HF/HC diet. Intestines were dissected from 35 larvae per group 20 h postfeeding and pooled for lipid extraction. The cholesterol BODIPY FL-C12 peak is present in the HPLC-fluorescence trace from intestines of larvae in the vehicle control group (upper chromatogram), and absent in the trace from intestines of ezetimibe-treated larvae (lower chromatogram). Results are representative of two samples per group.