Fig. 1.

Growth and propagation of primary murine intestinal enteroids. A: Duodenal crypts were plated in 3D culture in Matrigel at day 0 and were provided complete basal growth medium. Crypts reform tight junctions by day 1 and mature enteroids form at days 7–10. B: Caco-2 cells in 2D culture maintained through day 17 post confluence. C: Mature enteroids passaged at maturity 16 times maintain the phenotype of freshly isolated crypts and enteroids. Images were taken at 20× magnification on a Zeiss Axiovert 40C inverted light microscope and are representative of n = 12 unique enteroid lines (all C57Bl/6J WT).