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. 2017 Apr 28;58(5):853–865. doi: 10.1194/jlr.M071340

Fig. 2.

Fig. 2.

Analysis of differentiation markers of murine duodenal enteroids throughout the culture period. A, B: Percent mRNA expression in whole tissue versus freshly isolated crypts, enteroids in culture for 4 days, and mature enteroids in culture for 7–10 days [ALPI, VIL1, and KRT20 (A) and KRT18 (B)]. Results are mean ± SEM. *P < 0.05 compared with duodenum; n = 3–6 unique enteroid lines (all C57Bl/6J WT). C, D: WT enteroids were grown to maturity in basal growth medium, or differentiated, and differentiation marker mRNA expression was assessed in comparison to whole tissue (100%): LGR5, KRT18, KRT20, and TFF3 (C) and, ALPI, KLF4, VIL1, SI (D). Results are mean ± SEM. *P < 0.05 compared with basal enteroids; n = 3–6 unique enteroid lines (all C57Bl/6J WT).