Fig. 4.

Enteroids synthesize and secrete TAG from FA precursors. A: Genomic DNA content of parallel cultures of Caco-2 cells and enteroids. B: Secretion of TAG into media from nondifferentiated enteroid cultures and 17 day post confluent Caco-2 cells. C: Incorporation of BODIPY-C12 FFA into enteroid lumen during 2 h treatment with BODIPY-FFA micelles. D: Enteroids were treated with 400 uM OA:BSA or BSA alone, and 0.5 uCi [³H]OA and the conversion to [³H]TAG was assessed after Folch extraction in both the media and the cellular lipid contents. E: Enteroids were cultured under basal and differentiated conditions and treated with lipid as in (D). F: Enteroids and Caco-2 cells were treated as in (D) and total [³H]TAG in media and cells was assessed. Enteroids and Caco-2 cells were treated with mixed micelles containing [³H]OA (G) and [¹⁴C]cholesterol (H), and TAG and cholesterol accumulation was measured after Folch extraction. Results are mean ± SEM. *P < 0.05, comparing Caco-2 to day 10 enteroids [as in (B)], comparing enteroids to Caco-2 with same treatment (F); n = 3 unique enteroid lines (all C57Bl/6J WT).