Fig. 6.

Enteroids derived from hu-apoC-III Tg mice secrete less TAG and smaller chylomicrons. A: Enteroids derived from hu-apoC-III Tg mice express human apoC- III mRNA, WT enteroids do not. B: Basal secretion of TAG into media. C: Enteroids and Caco-2 cells were treated with mixed micelles containing [³H]OA and the rate of TAG accumulation was measured after Folch extraction (and compared directly to rate at which Caco-2 cells accumulate [³H]TAG. Results are mean ± SEM. *P < 0.05, comparing WT to hu-apoC-III Tg enteroids. D: Chylomicrons were isolated and sized as in Fig. 5B. E: Relative CD36 mRNA expression in WT and hu-apoC-III Tg enteroids. Results are mean ± SEM. *P < 0.05; n = 3–6 unique enteroid lines for WT and transgenic mice.