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. 2017 Apr 28;58(5):866–875. doi: 10.1194/jlr.M071357

Fig. 6.

Fig. 6.

miR-34a decreased SIRT1, MET, and KLF4 protein expression. A: LNA-34a decreased miR-34a levels by 91% in Mz-ChA-1 cells 24 h after transfection. B: Mz-ChA-1 or KMCH cells were transfected with negative control A LNA (Neg Con), microRNA mimic (miR-34a), or anti-miR-34a (LNA-34a) for 24 h. SIRT1, MET, and KLF4 expression was detected by immunoblot in whole-cell lysates. C, D: SIRT1 and MET loss in response to PA was measured in cells transfected with negative control A LNA (Neg Con) or miR-34a antagonist (LNA-34a). E: Lipoapoptosis in response to a low PA stimulus was tested in the presence of DMSO vehicle, Su11274 MET inhibitor (2 μM), EX527 SIRT1 inhibitor (100 μM), or both inhibitors. Apoptosis was assessed by caspase 3/7 activity. **P < 0.01; t-test in (A), ANOVA with Bonferroni post hoc correction in (E).