Fig. 7.

Cholangiocyte lipoapoptosis in animal model of NASH. A: A Masson trichrome-stained liver section from HFHS-fed mice showed enhanced lipid droplet and collagen accumulation compared with chow-fed control (Con) mice. B: Total RNA was isolated from flash-frozen liver tissue and miR-34a levels were measured relative to RNU6. C: Cholangiocytes were identified by pan-keratin staining and apoptotic nuclei were identified in the same section by TUNEL reaction. TUNEL-positive cholangiocytes are indicated as a percentage of total cholangiocyte nuclei. A total of 900–3,000 cholangiocytes were scored per sample. Data represent the mean ± SEM for n = 4 mice for each group. *P < 0.05, t-test.