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ACSL1 and ACSL4 overexpressed in COS7 cells use both EETs and HETEs as substrates. ACS activity with 5 μM final substrate concentrations of AA, 8,9-EET, 11,12-EET, 14,15-EET, 5-HETE, 12-HETE, and 15-HETE. Error bars reflect SEM from three separate experiments. S.A., specific activity.
