TABLE 1.
Vmax values from purified F-ACSLs with various substrates
| Vmax (μmol/min/mg) | |||||
| 16:0 | 18:0 | 18:1 | 18:2 | 20:4 | |
| F-ACSL1 | 3,754 ± 100a | 2,874 ± 74 | 2,089 ± 108 | 1,635 ± 33 | 3,363 ± 104 |
| F-ACSL3 | 2,763 ± 98a | 2,099 ± 33 | 2,109 ± 52 | 1,394 ± 31 | 2,627 ± 44 |
| F-ACSL4 | 4451 ± 60 | 2,737 ± 45 | 2,366 ± 79 | 1,231 ± 44 | 7,180 ± 229a |
| F-ACSL5 | 5,053 ± 209a | 2,164 ± 39 | 3,726 ± 44 | 1,351 ± 35 | 1,880 ± 49 |
| F-ACLS6 | 3,993 ± 105a | 1,725 ± 31 | 2,238 ± 35 | 1,763 ± 55 | 1,682 ± 69 |
Vmax values derived from kinetics data from Fig. 7 where data points are the mean of determinations from proteins obtained in three independent experiments.
Indicates the substrate with which the greatest ACS activity is achieved for each F-ACSL.