TABLE 2.
Km values from purified F-ACSLs with various substrates
| Km (μM) | |||||
| 16:0 | 18:0 | 18:1 | 18:2 | 20:4 | |
| F-ACSL1 | 2.7 ± 0.4 | 6.6 ± 0.7 | 6.7 ± 1.4 | 5.0 ± 0.4 | 6.3 ± 0.8 |
| F-ACSL3 | 4.0 ± 0.7 | 3.1 ± 0.2 | 5.3 ± 0.6 | 7.3 ± 0.6 | 4.3 ± 0.3 |
| F-ACSL4 | 4.5 ± 0.3 | 2.9 ± 0.2 | 16.7 ± 1.9 | 4.0 ± 0.6 | 11.4 ± 1.3 |
| F-ACSL5 | 6.5 ± 1.1 | 4.4 ± 0.4 | 6.3 ± 0.3 | 5.8 ± 0.6 | 1.8 ± 0.3 |
| F-ACLS6 | 3.0 ± 0.4 | 4.4 ± 0.4 | 4.4 ± 0.3 | 6.0 ± 0.8 | 2.9 ± 0.6 |
Km values derived from kinetics data from Fig. 7 where data points are the mean of determinations from proteins obtained in three independent experiments.