TABLE 3.
Vmax values from overexpressed ACSL1 and ACSL4 COS7 membranes with various substrates
| Vmax (μmol/min/mg) | |||||
| 16:0 | 18:0 | 18:1 | 18:2 | 20:4 | |
| COS7-ACSL1 | 3,068 ± 54a | 2,342 ± 74 | 2,607 ± 63 | 2,525 ± 75 | 1,745 ± 48 |
| COS7-ACSL4 | 1,990 ± 69 | 1,240 ± 29 | 466 ± 11 | 1,011 ± 30 | 4,339 ± 102a |
Vmax values derived from kinetics data from Fig. 8 where data points are the mean of determinations from proteins obtained in three independent experiments.
a
Indicates the substrate with which the greatest ACS activity is achieved for each COS7 ACSL.