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Confirmation of 20β-HSDH activity from B. scardovii ATCC BAA-773 (A) and steroid-17,20-desmolase activity in P. lymphophilum ACS-093-V-SCH5 (B). Each steroid substrate (50 μM) was incubated with both B. scardovii in BHI medium and P. lymphophilum in Gifu anaerobic medium for 48 h. The products were extracted and analyzed using HPLC equipped with a C-18 analytical column (Capcell Pak c18). Peak retention times and peak areas of samples were compared with retention times of standards.
