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. 2017 Apr 28;37(2):BSR20170182. doi: 10.1042/BSR20170182

Figure 6. Mechanism study of D. genkwa extractives in HSF cells after treatment with TNF-α.

Figure 6

(A) qRT-PCR analysis of expression levels of COL1A1, COL3A1, Timp-1, MMP-3, Cyclin D1 and c-fos, *P<0.05 compared with controls or TNF-α group. Values are means of three replicates. (B) The protein levels of COL1A1, COL3A1, Timp-1, MMP-3, Cyclin D1 and c-fos in HSF cells. (C) Western blotting analysis of MEK1/2, p-MEK1/2, ERK1/2 and p-ERK1/2. The root extractives obviously increased the levels of COL1A1, COL3A1, Timp-1, Cyclin D1, c-fos, MEK1/2, p-MEK1/2, ERK1/2 and p-ERK1/2 and decreased MMP-3 level affected by TNF-α.