Table 1.
Nucleotide sequences of primers used in the present study
| Primer name | Sequences (5′–3′) | Restriction sites |
|---|---|---|
| hGHpA-F | GGAACCTGCAGGGATCCACGGGTGGCATCCCTGT | PstI, BamHI |
| hGHpA-R | GGAACCTCGAGAAGGACAGGGAAGGGAG | XhoI |
| iRFP-F | GGAACGTCGACGCCACCATGGCGGAAGGATCCGT | SalI |
| iRFP-R | GGAACAGATCTTCACTCTTCCATCACGC | BglII |
| MCS+ | GATCTGTACACTAGTTTAAACCGGTCGACGGWCCGTACGGCGCGCCTAGGGCCCGGCCGGCC TTAAGCGCTAGCGATC | BsrGI, SpeI, PmeI, AgeI, RsrII, BsiwI, AscI, AvrII, ApaI, SfiI, FseI, AfeI, NheI, BmtI |
| MCS- | GATCGATCGCTAGCGCTTAAGGCCGGCCGGGCCCTAGGCGCGCCGTACGGWCCGTCGACC GGTTTAAACTAGTGTACA | BsrGI, SpeI, PmeI, AgeI, RsrII, BsiwI, AscI, AvrII, ApaI, SfiI, FseI, AfeI, NheI, BmtI |
| CMV promoter-F | GGAACAGATCTACGCGTGGAGCTAGTTAT | BglII |
| CMV promoter-R | GGAACAGATCTTCCCAATTCTTTGCCAAAGT | BglII |
| TRAIL-F | GGAACAGCGCTGCCACCATGGCTATGATGGAGGT | AfeI |
| TRAIL-R | GGAACAGATCTTTAGCCAACTAAAAAGG | AfeI |