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. 2017 Apr 28;37(2):BSR20170251. doi: 10.1042/BSR20170251

Figure 2. Expression and purification of WT and mutant forms of MBP-CHIP.

Figure 2

(A) The expression of MBP-CHIP proteins of both WT and mutant forms at time 0 and 24 h after induction of protein expression by 0.5 mM IPTG in BL21 E. coli cells. (B) Purified WT and mutant forms of MBP-CHIP after Ni-NTA purification procedure. (C) WT MBP-CHIP fusion protein cleavage, using TEV protease to MBP-CHIP mass ratio of 1:10 and an incubation time of 2 h. All samples were analyzed by SDS/PAGE (10% gel) and Coomassie Blue staining.