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. 2017 Apr 28;37(2):BSR20170251. doi: 10.1042/BSR20170251

Figure 4. Limited proteolysis by trypsin of WT and mutant CHIP proteins.

Figure 4

The proteolytic susceptibility of MBP-free CHIP proteins was explored for WT and mutants using trypsin for protein digestion. (A) Proteins were detected by SDS/PAGE and SYBRO Ruby staining after being subjected to trypsin proteolysis for various time periods (0–30 min). (B) Full-length proteins were quantified by ImageJ software, and the data for the average of three individual experiments were plotted against time. Each time point represents the mean of three readings conducted on three separate days ± SD (n=3).